Genomic Analysis Test
NEXJENNER SERVICE
We provide a variety of genome analysis services with the know-how of experts with 20 years of history in the Korean genome analysis market.
We provide customized services to researchers who need genome analysis.
Nexjenner Co., Ltd. provides integrated consulting at all stages from research design, including DNA sequencing, transcriptome sequencing, metagenome sequencing, and SNP genotyping, to sample preparation, genome analysis testing, statistical processing, and paper writing.
Genome Sequencing
Next Generation Sequencing (NGS) is a technology that produces large amounts of data in a short period of time and can be used for various research purposes targeting humans, animals, plants,
and microorganisms.
Human Exome Sequencing
· Read Mapping
· Variant calling (SNV, indels)
· Variant annotation
· Seq Depth > 50x
· DNA 1㎍ (Conc. ≥ 50ng/㎕)
· 260/280 = 1.6~2.2
Target DNA Sequencing
· Read Mapping- Variant calling (SNV, indels)
· Variant annotation
· Clinical annotation
· Seq Depth > 100x
· 0.5~1㎍ (Conc. ≥ 100ng/㎕)
· 260/280 = 1.6~2.2
Transcriptome Sequencing
It is possible to analyze differences in gene expression levels between normal and control groups, novel genes, and fusion genes from various samples such as human, mouse, animals, and plants. It can be used to see differences in gene expression after sample treatment or to study the function of genes related to diseases.
RNA Sequencing
· Reference alignment
· Abundance Estimation
· Differential expressed genes (DEGs)
· Novel gene, fusion genes
· Seq Depth 15-20M Reads
· Total RNA 1㎍ (Conc. ≥ 100ng/㎕)
· RIN ≥ 7
· DNA-free
Small RNA Sequencing
· Reference alignment
· Abundance Estimation
· Discovery of novel/known miRNA
· data size 5M Reads
· Total RNA 1㎍ (Conc. ≥ 100ng/㎕)
· RIN ≥ 7
· DNA-free
Ampliseq Transcriptome Sequencing – Human/ Mouse
· Diffential expressed genes
· data size 7-10M Reads
· Total RNA 0.1㎍ (Conc. ≥ 10ng/㎕)
· FFPE> 10ng
· RIN ≥ 7, DNA-free
Hierarchical Clustering
Scatter plot
PCA plot
Metagenome Sequencing
Identify and compare bacteria, fungi, and more in different samples. Microbiome analysis of bacteria that are difficult to culture is possible, allowing phylogenetic comparison of community diversity.
16S rRNA Taxonomic
Sequencing (v3-v4)
· Taxonomic profiling
· Comparison analysis of microbial community
· Diversity statistics (alpha-, beta- diversity)
· Seq Depth > 50,000 reads
· gDNA>100ng (Conc. 20ng/㎕)
· 260/280 ratio 1.8~2.0
AmpliSeq Metagenome
Sequencing
· Diversity statistics (alpha-, beta- diversity)
· Species of Cancer, immunological and
gastrointestinal bacteria
· gDNA>100ng (Conc. 20ng/㎕)
· 260/280 ratio 1.8~2.0
Shotgun Metagenome
Sequencing
· Remove host sequences
· Metagenome assembled genomes
· Prediction genomic region
· Gene annotation
· Taxonomy assign
· gDNA>2㎍ (Conc. ≥ 100ng/㎕)
· 260/280 ratio 1.8~2.0
AmpliSeq Microbiome Health Research Kit
AmpliSeq Microbiome Health Research Kit
Akkermansia muciniphila
Anaerococcus vaginalis
Atopobium parvulum
Bacteroides fragilis
Bacteroides nordii
Bacteroides thetaiotaomicron
Bacteroides vulgatus
Barnesiella intestinihominis
Bifidobacterium adolescentis
Bifidobacterium animalis
Bifidobacterium bifidum
Bifidobacterium longum
Blautia obeum
Borrelia burgdorferi
Campylobacter concisus
Campylobacter curvus
Campylobacter gracilis
Campylobacter hominis
Campylobacterjejuni
Campylobacter rectus
Chlamydia pneumoniae
Chlamydia trachomatis
Citrobacter rodentium
Cloacibacillus porcorum
Clostridium difficile
Collinsella aerofaciens
Collinsella stercoris
Desulfovibrio alaskensis
Dorea formicigenerans
Enterococcus faecalis
Enterococcus faecium
Enterococcus gallinarum
Enterococcus hirae
Escherichia coli
Eubacterium limosum
Eubacterium rectale
Faecalibacterium prausnitzii
Fusobacterium nucleatum
Gardnerella vaginalis P
Gemmiger formicilis
Helicobacter bi!is
Helicobacter bizzozeronii
Helicobacter hepaticus
Helicobacter pylori
Holdemania filiformis
Klebsiella pneumoniae
Lactobacillus acidophilus
Lactobacillus delbrueckii
Lactobacillus johnsonii
Lactobacillus murinus
Lactobacillus reuteri
Lactobacillus rhamnosus
Lactococcus tactis
Mycoplasma fermentans
Mycoplasma penetrans
Parabacteroides distasonis
Parabacteroides merdae
Peptostreptococcus
arvimonas micra
Peptostreptococcus anaerobius
Peptostreptococcus stomatis
Phascolarctobacterium faecium
Porphyromonas gingivalis
Prevotella copri
Prevotella histicola
Propionibacterium acnes
(Cuubacterium acnes)
Proteus mirabilis
Roseburia intestinalis
Ruminococcus bromii
Ruminococcus gnavus
Slackia exigua
Streptococcus gallolyticus
Streptococcus infantarius
Veil/oneffa parvufa
SNP Genotyping
We provide SNP Genotyping Microarray and Custom Genotyping services that can analyze millions of SNPs simultaneously. In addition to full-length human genome analysis and confirmation of chromosomal abnormalities, animal and plant microarray analysis is possible.
DNA MicroArray
-Illumina System
· Genetic Association Study (Helixtree)
· Genome Wide Association Study
(Q-Q plot, Manhattan plot etc.)
· Chromosome aberration
· CNV region define
· DNA1㎍ (Conc. ≥ 50ng/㎕)
· 260/280 = 1.6~2.2
Custom Genotyping
- Fluidigm System
· Genetic Association
(Additive, codominant, recessive model)
· LD, haplotype analysis
· Conditional analysis
· 0.5~1㎍ (Conc. ≥ 20ng/㎕)
· 260/280 = 1.6~2.2
We provide validation services for mutations discovered through NGS.
Realtime PCR
· SNP genotyping
· Gene expression
· Copy number analysis
· 0.1~0.5㎍ (Conc. ≥ 5ng/㎕)
· 260/280 = 1.6~2.2
Sanger Sequencing
· NGS validation
· difficult region Variant calling
· 0.5~1㎍ (Conc. ≥ 20ng/㎕)
· 260/280 = 1.6~2.2
Main Equipment
· Sequencing : Lon GeneStudio S5 Prime/ Lon Chef/ Miseq
· SNP MicroArray & Genotyping : QuantStudio 3/ EP1/ ISCAN/ 3730 Genetic Analyzer
